Vol. 6 No. 01 (2023)
Original Article

Efficacy of P16 in Biopsy Samples in the Diagnosis of High Grade Cervical Intraepithelial Neoplasia — An Observational Study

Most Israt Jahan
Associate Professor (C.C), Department of Pathology, M Abdus Rahim Medica Coege, Dinajpur, Bangladesh
Md Mahbub Morshed
Assistant Professor, Department of Critical Care Medicine, M Abdus Rahim Medica Coege, Dinajpur, Bangladesh
Nure Jannat Ferdousi Ara
Assistant Professor, Department of Pathology, M Abdus Rahim Medica Coege, Dinajpur, Bangladesh
Shamima Yasmin
Associate Professor (C.C), Department of Biochemistry, M Abdus Rahim Medica Coege, Dinajpur, Bangladesh
Shabnam Akhter
Associate Professor , Department of Pathology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh

Find this Article on:

Abstract

Introduction: Invasive cervical cancer is preceded by a precancerous lesion known as cervical intraepithelial neoplasia (CIN). The majority of low-grade CIN lesions (CIN-1) spontaneously resolve without any medication, with only a tiny percentage progressing to high-grade lesions (CIN 2 and CIN 3) and cervical cancer. Objective: To evaluate the performance of P16 in the diagnosis of high-grade cervical intraepithelial neoplasia in biopsy samples at a Bangladeshi tertiary care facility. Methods and Materials: This retrospective observational study was conducted at Bangabandhu Sheikh Mujib Medical University in Dhaka. The study focused on 75 cases with histological diagnoses of CIN 2 and CIN 3/CIS. These cases were selected from the pathology department's archive, covering the period from June 2014 to December 2015. The fresh H&E sections and previous H&E sections were examined. 47 cases were ultimately available for examination after meeting the selection criterion. Of these 47 instances, 11 had CIN 3, and 36 had a CIN 2 diagnosis. All underwent P16 immunostaining. Results: Out of the 47 cases, 36 were diagnosed as CIN 2 (76.59 %) and 11 cases as CIN 3 (23.40%). The age of the study patients ranged from 25 to 70 years with a mean age of 41.4 +11.5 years. Around 60% of the study patients belonged to the age group 31- 50 years. In all 11 (100%) of the CIN 3 cases, P16 immunostain was found positive. 10 (27.77%) of the CIN 2 cases had positive P16 immunostain results. Conclusion: Because of the presence of immature squamous metaplasia, reactive epithelial alterations, atrophy, or tangential sectioning, the diagnosis of CIN 2 exhibits intraobserver and interobserver variability.

Published 28-11-2023

Keywords

  • CIN 2 and 3,
  • P16 immunostain,
  • Invasive cervical cancer

How to Cite

1.
Jahan MI, Morshed MM, Ara NJF, Yasmin S, Akhter S. Efficacy of P16 in Biopsy Samples in the Diagnosis of High Grade Cervical Intraepithelial Neoplasia — An Observational Study . The Insight [Internet]. 2023 Nov. 28 [cited 2024 Jul. 25];6(01):285-91. Available from: https://bdjournals.org/index.php/insight/article/view/363