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Keywords
ß-thalassemia trait
Hemoglobin electrophoresis
NESTROFT
How to Cite
Abstract
Background: Worldwide the hemoglobin disorders are the most common clinically serious single gene disorders and are often regarded as incurable and expensive to treat. Objective: To find out sensitivity, specificity and predictive values of Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) for detection of carriers of ß-Thalassemia. Methods: This descriptive analytic type of study was carried out in the Department of Pediatrics of Sylhet MAG Osmani Medical College Hospital, Sylhet, from July 2006 to June 2008. A total of 70 samples, 35 cases and 35 suitably matched controls were enrolled. In this study, siblings, parents and first degree cousins of patients of β-thalassemia major who had Hemoglobin A2>3.6%+HbA on hemoglobin electrophoresis were selected randomly as cases and the same of whom had Hemoglobin A2 (2.5±0.2)+ HbA on hemoglobin electrophoresis were selected as controls. Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) was done from the cases and controls. Results: Sensitivity and specificity of NESTROFT in the diagnosis of carriers of β-thalassemia were found 94.3 % and 88.6 % respectively. The positive predictive value and negative predictive value of NESTROFT in the diagnosis of carriers of βthalassemia were found 89.1% and 94% respectively. Conclusion: NESTROFT is a valid screening test for detection of ß-thalassemia trait andNESTROFT may be used as an alternative to hemoglobin electrophoresis to detect ß-thalassemia trait in Bangladesh.
References
Weatherall DJ, Clegg JB. Inherited haemoglobin disorders: an increasing global health problem. Bulletin of the World Health Organization. 2001;79: 704–712.
Shivashankara AR, Jailkhani R, Kini A. Hemoglobinopathies in Dharwad, North Karnataka: a hospital-based study. Journal of Clinical and Diagnostic Research .2008; 2; 593-599.
Angastiniotis M, Modell B. Global epidemiology of hemoglobin disorders. Ann N Y Acad Sci.1998; 850:251-269
Weatherall DJ, Clegg TB, Higgs DR, Wood WG. The hemoglobinopathies. In: Scriver CR, Beaudet AL, Sly WS, Valle D. (Eds.). The Metabolic and Molecular Bases of Inherited Diseases, 8th Edition. New York: McGraw Hill; 2001: 4571-4636.
Fucharoen G, Sanchaisuriya K, Sae-ung N, Dangwibul S, Fucharoen S. A simplified screening strategy for thalassaemia and haemoglobin E in rural communities in south-east Asia. Bulletin of the World Health Organization. 2004; 82(5): 364-372.
Organization WH et al. Management of haemoglobin disorders: report of a joint WHO-TIF meeting, Nicosia, Cyprus, 16-18 November 2007. 2008.
Khan WA, Banu B, Amin SK. Prevalence of ß-Thalassemia trait and Hb-E trait in Bangladeshi school children and health burden of thalassemia in our population. Dhaka Shishu (children) Hospital Journal. 2005; 21(1): 1-7.
Rahman SA, Jamal CY. Congenital Hemolytic Anemia in Bangladesh: Types and Clinical Manifestations. Indian Pediatrics. 2002; 39:574-577.
Hasan MK, Haque O, Rubaiyat KA, BarshanAD, Talukder SI.Clinical Presentation and Electrophoretic Patterns of Hereditary Haemoglobin Disorders in Adults, a Study at Dinajpur Medical College Hospital. Dinajpur Med Col J. 2013;6:167-71.
Farhana DT, Quamrun N, Subhagata C. J Bangladesh Coll Phys Surg. 2009; 27: 148-54.
Maheshwari M, Arora S, Kabra M, Menon PSN. Carrier screening and prenatal diagnosis of β-thalassaemia. Indian Pediatrics.1999; 36:1119-1125.
Manglani M, Lokeshwar MR, Vani VG, Bhatia N, Mhaskar V. 'NESTROFT'-an effective screening test for beta thalassemia trait. Indian Journal of Pediatrics. 1997; 34: 702-707.
Begum JA, Amin SK, Khan WA, Selimuzzaman M, Sharmin S, Hossain B. Evaluation of naked eye single tube red cell osmotic fragility test (NESTROFT) in detecting ß-Thalassemia trait. Dhaka Shishu Hospital Journal. 2005; 21(2): 44-48.
Mehta BC. NESTROFT: A screening test for beta thalassemia trait. Indian J Med Sc. 2002; 56: 537-44.
Raghavan K, Lokeshwar MR, Birewar N, Nigam V, Manglani MV, Raju NB. Evaluation of naked eye single tube red cell osmotic fragility test in detecting beta-thalassemia trait. Indian pediatrics.1991; 28(5): 469-72.
Kattamis C, Efremovt G, Pootrakul S. Effectiveness of one tube osmotic fragility screening in detecting βthalassaemia trait. Journal of Medical Genetics.1981; 18:266-270.
Thomas S, Srivastava A, Jeyaseelan L, Dennison D, Chandy M. NESTROFT as a screening test for the detection of thalassemia and common haemoglobinopathies- An evaluation against a high performance liquid chromatographic method. Ind J Med Res. 1996; 104:194–7.
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